The adapter was replaced, saturated fat sportline

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The adapter was replaced, both stopcocks were closed, and the magnetic stirrer started. Fifty mL of air sportline was withdrawn from the sealed chamber and replaced with sportline 50 mL of cyclopropane withdrawn from a gas cylinder. Before injection, the syringe needle was purged to atmospheric pressure by placing it under water until bubbling of cyclopropane from the syringe had stopped. After two and one-half hours, 20-wL samples of chamber gas were removed every 10 to 15 min and analyzed with a Hewlett Packard 5890A sportline Gas Chromatograph (FID, 1.8 m glass column packed with 0.19% picric acid in Carbopack [Supelco Inc., Bellefonte, Pennsylvania], 100 degC isothermal). Sampling continued until the SD of the mean of three samples was less than 0.75%. Cyclopropane concentration was calibrated using external standards that were made by injecting 10 to 18 mL cyclopropane into 1L volumetric flasks.
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