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| body composition, fish oil, accumeasure calipers, ball, fat, weight loss diet, pr nutrition, midsection, iss research, heart disease, tape measures, lifestyle, slimming, compare prices, food, cardio zone, lancome, ear thermometer, | A Teflon gasket, smeared with a thin layer of glycerol, was positioned d between the lid and base of the chamber and held firmly in place with a clamp that applied pressure d completely around the flange of the lid and base. After the d lid was tightened, we temporarily removed the adapter containing the manometer from the lid, and flushed 100% O^ sub2^ into the chamber through this port for about 2 to 3 min. The adapter was replaced, both stopcocks were closed, and the magnetic stirrer started. Fifty mL of air was withdrawn from the sealed chamber and replaced with 50 mL of cyclopropane withdrawn from a gas cylinder. Before injection, the syringe needle was purged to atmospheric pressure by placing it under water until bubbling of cyclopropane from the syringe had stopped. After two and one-half hours, 20-wL samples of chamber gas were removed every 10 to 15 min and analyzed with a Hewlett Packard 5890A Gas Chromatograph (FID, 1.8 |
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| Chamber gas samples (20 muL) were obtained from one stopcock for cyclopropane analysis, and 100% oxygen or room air was injected into the chamber through the other stopcock to restore lowered chamber pressure (created by the animal's consumption iss research of oxygen and iss research the chemical absorption of its CO^ sub2^ in the chamber) to atmospheric pressure. A U-shaped glass manometer, which contained glycerol and measured chamber pressure, was attached with Teflon tape to the glass adapter, and a thermocouple wire, which measured chamber-gas temperature, entered the side arm of the adapter and extended 2 cm into the iss research chamber. The manometer detected decreases in chamber pressure as the animal consumed oxygen. Material surfaces within the chamber included glass, aluminum, Teflon, and glycerol. Cyclopropane is not absorbed by these materials but is soluble in commonly used laboratory materials such as rubber, stopcock grease, and plastic. |
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